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xt dna library prep kit v2 for indexing  (Illumina Inc)


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    Structured Review

    Illumina Inc xt dna library prep kit v2 for indexing
    Xt Dna Library Prep Kit V2 For Indexing, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 11826 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/xt+dna+library+prep+kit+v2+for+indexing/Nextera+XT+DNA+Library+Preparation+Kit/pmc12137813-288-11-21
    Average 99 stars, based on 11826 article reviews
    xt dna library prep kit v2 for indexing - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: The Epidome - a species-specific approach to assess the population structure and heterogeneity of Staphylococcus epidermidis colonization and infection
    Article Snippet: The duplex amplification PCR was carried out using 2x KAPA HiFi (Kapa Biosystems) with 0.2 μM of each primer and 10 μL template in a final volume of 25 μL on a 2720 Thermal Cycler (Applied Biosystems) using the following PCR conditions: 3 min at 95 °C, 25 cycles of 20s at 98 °C, 15 s at 60 °C and 45 s at 72 °C with a final extension step of 5 min at 72 °C. .. The indexing PCR was carried out using 2x KAPA HiFi, Nextera XT DNA Library Prep Kit v2 for indexing (Illumina) and with 2 μL amplicon product from the amplification PCR in final volume of 25 μL on a 2720 Thermal Cycler (Applied Biosystems) using the following conditions: 3 min at 95 °C, 20 cycles of 20s at 98 °C, 15 s at 55 °C and 45 s at 72 °C, and final 5 min elongation step at 72 °C. .. After indexing, post-PCR cleanup was performed using a 1:1 ratio of AMPure XP beads (Beckman Coulter) following manufacturer’s instructions before quantification using AccuClear Ultra High Sensitivity dsDNA Quantification Kit (Biotium) following manufacturer’s instructions.

    Article Title: The Epidome - a species-specific approach to assess the population structure and heterogeneity of Staphylococcus epidermidis colonization and infection.
    Article Snippet: The duplex amplification PCR was carried out using 2x KAPA HiFi (Kapa Biosystems) with 0.2 μM of each primer and 10 μL template in a final volume of 25 μL on a 2720 Thermal Cycler (Applied Biosystems) using the following PCR conditions: 3 min at 95 °C, 25 cycles of 20s at 98 °C, 15 s at 60 °C and 45 s at 72 °C with a final extension step of 5 min at 72 °C. .. The indexing PCR was carried out using 2x KAPA HiFi, Nextera XT DNA Library Prep Kit v2 for indexing (Illumina) and with 2 μL amplicon product from the amplification PCR in final volume of 25 μL on a 2720 Thermal Cycler (Applied Biosystems) using the following conditions: 3 min at 95 °C, 20 cycles of 20s at 98 °C, 15 s at 55 °C and 45 s at 72 °C, and final 5 min elongation step at 72 °C. .. After indexing, post-PCR cleanup was performed using a 1:1 ratio of AMPure XP beads (Beckman Coulter) following manufacturer’s instructions before quantification using AccuClear Ultra High Sensitivity dsDNA Quantification Kit (Biotium) following manufacturer’s instructions.

    Article Title: The Epidome - A species-specific Approach to Assess the Population Structure and Heterogeneity of Staphylococcus Epidermidis Colonization and Infection
    Article Snippet: PCR and sequencing Page 7/20 The duplex ampli cation PCR was carried out using 2x KAPA HiFi (Kapa Biosystems) with 0.2 μM of each primer and 10 μL template in a nal volume of 25 μL on a 2720 Thermal Cycler (Applied Biosystems) using the following PCR conditions: 3 min at 95 ̊C, 25 cycles of 20s at 98 ̊C, 15s at 60 ̊C and 45s at 72 ̊C with a nal extension step of 5 min at 72 ̊C. .. The indexing PCR was carried out using 2x KAPA HiFi, Nextera XT DNA Library Prep Kit v2 for indexing (Illumina) and with 2 μL amplicon product from the ampli cation PCR in nal volume of 25 μL on a 2720 Thermal Cycler (Applied Biosystems) using the following conditions: 3 min at 95 ̊C, 20 cycles of 20s at 98 ̊C, 15s at 55 ̊C and 45s at 72 ̊C, and nal 5 min elongation step at 72 ̊C. .. After indexing, post-PCR cleanup was performed using a 1:1 ratio of AMPure XP beads (Beckman Coulter) following manufacturer’s instructions before quanti cation using AccuClear Ultra High Sensitivity dsDNA Quanti cation Kit (Biotium) following manufacturer’s instructions.

    Article Title: Does intervention with clindamycin and a live biotherapeutic drug containing Lactobacillus crispatus impact the reproductive outcome of IVF patients with abnormal vaginal microbiota: a randomised double-blind, placebo-controlled multicentre trial
    Article Snippet: Ampli cation of the 16S Illumina sequencing, Kapa HiFi HotStart polymerase ready mix (Sigma-Aldrich) was used with the primer sequences described above, but with adaptors with heterogeneity spacers for MiSeq indexing and sequencing as described in Table S4. .. The indexing PCR was carried out using 2x KAPA HiFi, Nextera XT DNA Library Prep Kit v2 for indexing (384 combinations) (Illumina) and with 2 μL amplicon from the ampli cation PCR in a nal volume of 25 μL on a 2720 Thermal Cycler using the following conditions: 3 min at 95 °C, 15 cycles of 20s at 98 °C, 15 s at 55 °C and 45 s at 72 °C, and nal 5 min elongation step at 72 °C. .. After indexing, post-PCR cleanup was performed using a 1:1 ratio of AMPure XP beads (Beckman Coulter) following manufacturer’s instructions before quanti cation using AccuClear Ultra High Sensitivity dsDNA Quanti cation Kit (Biotium) following manufacturer’s instructions.

    Article Title: Efficacy of clindamycin and LACTIN-V for in vitro fertilization patients with vaginal dysbiosis: a randomised double-blind, placebo-controlled multicentre trial
    Article Snippet: Amplification of the 16S Illumina sequencing, Kapa HiFi HotStart polymerase ready mix (Sigma-Aldrich) was used with the primer sequences described above, but with adaptors with heterogeneity spacers for MiSeq indexing and sequencing as described in Table . .. The indexing PCR was carried out using 2× KAPA HiFi, Nextera XT DNA Library Prep Kit v2 for indexing (384 combinations) (Illumina) and with 2 μL amplicon from the primary PCR in a final volume of 25 μL on a 2720 Thermal Cycler using the following conditions: 3 min at 95 °C, 15 cycles of 20 s at 98 °C, 15 s at 55 °C and 45 s at 72 °C, and final 5 min elongation step at 72 °C. .. After indexing, post-PCR cleanup was performed using a 1:1 ratio of AMPure XP beads (Beckman Coulter) following manufacturer’s instructions before quantification using AccuClear Ultra High Sensitivity dsDNA Quantification Kit (Biotium) following manufacturer’s instructions.

    Article Title: Efficacy of clindamycin and LACTIN-V for in vitro fertilization patients with vaginal dysbiosis: a randomised double-blind, placebo-controlled multicentre trial.
    Article Snippet: Amplification of the 16S Illumina sequencing, Kapa HiFi HotStart polymerase ready mix (Sigma-Aldrich) was used with the primer sequences described above, but with adaptors with heterogeneity spacers for MiSeq indexing and sequencing as described in Table S4. .. The indexing PCR was carried out using 2× KAPA HiFi, Nextera XT DNA Library Prep Kit v2 for indexing (384 combinations) (Illumina) and with 2μL amplicon from the primary PCR in a final volume of 25μL on a 2720 Thermal Cycler using the following conditions: 3min at 95 °C, 15 cycles of 20 s at 98 °C, 15 s at 55 °C and 45 s at 72 °C, and final 5min elongation step at 72 °C. .. After indexing, post-PCR cleanupwas performed using a 1:1 ratio of AMPureXP beads (Beckman Coulter) following manufacturer’s instructions before quantification using AccuClear Ultra High Sensitivity dsDNA Quantification Kit (Biotium) following manufacturer’s instructions.

    Amplification:

    Article Title: The Epidome - a species-specific approach to assess the population structure and heterogeneity of Staphylococcus epidermidis colonization and infection
    Article Snippet: The duplex amplification PCR was carried out using 2x KAPA HiFi (Kapa Biosystems) with 0.2 μM of each primer and 10 μL template in a final volume of 25 μL on a 2720 Thermal Cycler (Applied Biosystems) using the following PCR conditions: 3 min at 95 °C, 25 cycles of 20s at 98 °C, 15 s at 60 °C and 45 s at 72 °C with a final extension step of 5 min at 72 °C. .. The indexing PCR was carried out using 2x KAPA HiFi, Nextera XT DNA Library Prep Kit v2 for indexing (Illumina) and with 2 μL amplicon product from the amplification PCR in final volume of 25 μL on a 2720 Thermal Cycler (Applied Biosystems) using the following conditions: 3 min at 95 °C, 20 cycles of 20s at 98 °C, 15 s at 55 °C and 45 s at 72 °C, and final 5 min elongation step at 72 °C. .. After indexing, post-PCR cleanup was performed using a 1:1 ratio of AMPure XP beads (Beckman Coulter) following manufacturer’s instructions before quantification using AccuClear Ultra High Sensitivity dsDNA Quantification Kit (Biotium) following manufacturer’s instructions.

    Article Title: The Epidome - a species-specific approach to assess the population structure and heterogeneity of Staphylococcus epidermidis colonization and infection.
    Article Snippet: The duplex amplification PCR was carried out using 2x KAPA HiFi (Kapa Biosystems) with 0.2 μM of each primer and 10 μL template in a final volume of 25 μL on a 2720 Thermal Cycler (Applied Biosystems) using the following PCR conditions: 3 min at 95 °C, 25 cycles of 20s at 98 °C, 15 s at 60 °C and 45 s at 72 °C with a final extension step of 5 min at 72 °C. .. The indexing PCR was carried out using 2x KAPA HiFi, Nextera XT DNA Library Prep Kit v2 for indexing (Illumina) and with 2 μL amplicon product from the amplification PCR in final volume of 25 μL on a 2720 Thermal Cycler (Applied Biosystems) using the following conditions: 3 min at 95 °C, 20 cycles of 20s at 98 °C, 15 s at 55 °C and 45 s at 72 °C, and final 5 min elongation step at 72 °C. .. After indexing, post-PCR cleanup was performed using a 1:1 ratio of AMPure XP beads (Beckman Coulter) following manufacturer’s instructions before quantification using AccuClear Ultra High Sensitivity dsDNA Quantification Kit (Biotium) following manufacturer’s instructions.

    Article Title: The Epidome - A species-specific Approach to Assess the Population Structure and Heterogeneity of Staphylococcus Epidermidis Colonization and Infection
    Article Snippet: PCR and sequencing Page 7/20 The duplex ampli cation PCR was carried out using 2x KAPA HiFi (Kapa Biosystems) with 0.2 μM of each primer and 10 μL template in a nal volume of 25 μL on a 2720 Thermal Cycler (Applied Biosystems) using the following PCR conditions: 3 min at 95 ̊C, 25 cycles of 20s at 98 ̊C, 15s at 60 ̊C and 45s at 72 ̊C with a nal extension step of 5 min at 72 ̊C. .. The indexing PCR was carried out using 2x KAPA HiFi, Nextera XT DNA Library Prep Kit v2 for indexing (Illumina) and with 2 μL amplicon product from the ampli cation PCR in nal volume of 25 μL on a 2720 Thermal Cycler (Applied Biosystems) using the following conditions: 3 min at 95 ̊C, 20 cycles of 20s at 98 ̊C, 15s at 55 ̊C and 45s at 72 ̊C, and nal 5 min elongation step at 72 ̊C. .. After indexing, post-PCR cleanup was performed using a 1:1 ratio of AMPure XP beads (Beckman Coulter) following manufacturer’s instructions before quanti cation using AccuClear Ultra High Sensitivity dsDNA Quanti cation Kit (Biotium) following manufacturer’s instructions.

    Article Title: Does intervention with clindamycin and a live biotherapeutic drug containing Lactobacillus crispatus impact the reproductive outcome of IVF patients with abnormal vaginal microbiota: a randomised double-blind, placebo-controlled multicentre trial
    Article Snippet: Ampli cation of the 16S Illumina sequencing, Kapa HiFi HotStart polymerase ready mix (Sigma-Aldrich) was used with the primer sequences described above, but with adaptors with heterogeneity spacers for MiSeq indexing and sequencing as described in Table S4. .. The indexing PCR was carried out using 2x KAPA HiFi, Nextera XT DNA Library Prep Kit v2 for indexing (384 combinations) (Illumina) and with 2 μL amplicon from the ampli cation PCR in a nal volume of 25 μL on a 2720 Thermal Cycler using the following conditions: 3 min at 95 °C, 15 cycles of 20s at 98 °C, 15 s at 55 °C and 45 s at 72 °C, and nal 5 min elongation step at 72 °C. .. After indexing, post-PCR cleanup was performed using a 1:1 ratio of AMPure XP beads (Beckman Coulter) following manufacturer’s instructions before quanti cation using AccuClear Ultra High Sensitivity dsDNA Quanti cation Kit (Biotium) following manufacturer’s instructions.

    Article Title: Efficacy of clindamycin and LACTIN-V for in vitro fertilization patients with vaginal dysbiosis: a randomised double-blind, placebo-controlled multicentre trial
    Article Snippet: Amplification of the 16S Illumina sequencing, Kapa HiFi HotStart polymerase ready mix (Sigma-Aldrich) was used with the primer sequences described above, but with adaptors with heterogeneity spacers for MiSeq indexing and sequencing as described in Table . .. The indexing PCR was carried out using 2× KAPA HiFi, Nextera XT DNA Library Prep Kit v2 for indexing (384 combinations) (Illumina) and with 2 μL amplicon from the primary PCR in a final volume of 25 μL on a 2720 Thermal Cycler using the following conditions: 3 min at 95 °C, 15 cycles of 20 s at 98 °C, 15 s at 55 °C and 45 s at 72 °C, and final 5 min elongation step at 72 °C. .. After indexing, post-PCR cleanup was performed using a 1:1 ratio of AMPure XP beads (Beckman Coulter) following manufacturer’s instructions before quantification using AccuClear Ultra High Sensitivity dsDNA Quantification Kit (Biotium) following manufacturer’s instructions.

    Article Title: Efficacy of clindamycin and LACTIN-V for in vitro fertilization patients with vaginal dysbiosis: a randomised double-blind, placebo-controlled multicentre trial.
    Article Snippet: Amplification of the 16S Illumina sequencing, Kapa HiFi HotStart polymerase ready mix (Sigma-Aldrich) was used with the primer sequences described above, but with adaptors with heterogeneity spacers for MiSeq indexing and sequencing as described in Table S4. .. The indexing PCR was carried out using 2× KAPA HiFi, Nextera XT DNA Library Prep Kit v2 for indexing (384 combinations) (Illumina) and with 2μL amplicon from the primary PCR in a final volume of 25μL on a 2720 Thermal Cycler using the following conditions: 3min at 95 °C, 15 cycles of 20 s at 98 °C, 15 s at 55 °C and 45 s at 72 °C, and final 5min elongation step at 72 °C. .. After indexing, post-PCR cleanupwas performed using a 1:1 ratio of AMPureXP beads (Beckman Coulter) following manufacturer’s instructions before quantification using AccuClear Ultra High Sensitivity dsDNA Quantification Kit (Biotium) following manufacturer’s instructions.



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